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The FLIPR assay was carried out on FDSS/µMobile with ligand addition at 10?s with the binding assay and mAb1/isotype addition at 10?s followed by hCCL1 addition at three hundred?s with the Competitiveness binding assay, with a complete monitoring of 500?s. The excitation and emission wavelengths were being set at 485?nm and 525?nm, respectively. O